Table 1 Primer sequences for quantitative PCR (QPCR) used in this study
Gene/Symbol | Accession # | Orientation | Mouse-Specific Primer Sequences (5' to 3') |
|---|---|---|---|
RBP1 | NM_001081195 | Forward | GCAAAAGACGAACCCCAAAGCGGAC |
Reverse | TCCTGCGTGAGACACTTCTCTGTC | ||
Runx2 | NM_009820 | Forward | GCCGGGAATGATGAGAACTA |
Reverse | GGTGAAACTCTTGCCTCGTC | ||
Osterix | NM_130458 | Forward | GGAGGTTTCACTCCATTCCA |
Reverse | TAGAAGGAGCAAGGGGACAGA | ||
BMP2 | NM_007553 | Forward | ACGTCCTCAGCGAATTTGAG |
Reverse | GCCTGCGGTACAGATCTAGC | ||
Osteocalcin | NM_007541 | Forward | GCCATCACCCTGTCTCCTAA |
Reverse | GCTGTGGAGAAGACACACGA | ||
Alkaline Phos. | NM_007431 | Forward | TGAGCGACACGGACAAGA |
Reverse | GGCCTGGTAGTTGTTGTGAG | ||
BSP | NM_008318 | Forward | TTCCCAGGTGTGTCATTGAAGA |
Reverse | GGTATGTTTGCGCAGTTAGCAA | ||
TBP | NM_013684 | Forward | GCACTTCGTGCAAGAAATGCTG |
Reverse | CATAGCTCTTGGCTCCTGTGC | ||
Runx2: P1 | N/A | Forward | CCGGCCACTTCGCTAACTT |
Reverse | TGGTGCTCGGATCTACAGGAA | ||
Runx2: P2 | N/A | Forward | TCTGGAAAAAAAAGGAGGGACTATG |
Reverse | GGTGCTCGGATCCCAAAAGAA |