Figure 2

Alizarin Red staining reveals a significant delay in osteoblastic mineralization of the CalOB-ΔRBP1 cell line. The CalOB-Control (A) and CalOB-ΔRBP1 (B) cell lines were plated (n = 3) and allowed to differentiate in osteoblastic differentiation media for 0, 10, 17 and 31 days. The cells were subsequently stained using Alizarin Red. (C) Total RNA was extracted from identically treated CalOB-Control (solid line) and CalOB-ΔRBP1 (dotted line) cells and processed for real-time QPCR analysis (n = 3). The data are expressed as RBP1 expression relative to a TBP control and normalized to the day 0 CalOB-Control cell line. The data points represent the mean ± standard deviation. The asterisks represents statistical significance at the p < 0.01 level (ANOVA).