Table 1 Primers for qRT-PCT analysis
From: Characterization of differential properties of rabbit tendon stem cells and tenocytes
Gene | Size (bp) | Primers | Type | Tm | Gene Bank # | Ref. |
|---|---|---|---|---|---|---|
Collagen I | 81 | 5'-CTG ACT GGA AGA GCG GAG AGT AC-3' | Forward | 63°C | AY633663 | [14] |
| Â | Â | 5'-CCA TGT CGC AGA AGA CCT TGA-3' | Reverse | Â | Â | Â |
Collagen II | 84 | 5'-TGG GTG TTC TAT TTA TTT ATT GTC TTC CT-3' | Forward | 63°C | S83370 | [14] |
| Â | Â | 5'-GCG TTG GAC TCA CAC CAG TTA GT-3' | Reverse | Â | Â | Â |
Sox9 | 79 | 5'-AGT ACC CGC ACC TGC ACA AC-3' | Forward | 59°C | AY598935 | [14] |
| Â | Â | 5'-CGC TTC TCG CTC TCG TTC AG-3' | Reverse | Â | Â | Â |
Runx2 | 70 | 5'-TGA TGA CAC TGC CAC CTC TGA-3' | Forward | 58°C | AY598934 | [14] |
| Â | Â | 5'-GCA CCT GCC TGG CTC TTC T-3' | Reverse | Â | Â | Â |
GAPDH | 107 | 5'-ACT TTG TGA AGC TCA TTT CCT GGT A-3' | Forward | 63°C | L23961 | [14] |
| Â | Â | 5'-GTG GTT TGA GGG CTC TTA CTC CTT-3' | Reverse | Â | Â | Â |
PPARγ | 200 | 5'-TGG GGA TGT CTC ATA ATG CCA-3' | Forward | 59°C | AF013266 | [15] |
| Â | Â | 5'-TTC CTG TCA AGA TCG CCC TCG-3' | Reverse | Â | Â | Â |