Figure 3

Type 1 collagen culture effects on β-catenin accumulation in DD and PF-cells. PF cells (A) and DD cells (B) were cultured on Type 1 collagen coated slides (1.9 mg/ml) for 72 hours. Cells were fixed and stained with Oregon green phalloidin (green) and DAPI (blue) to label filamentous actin and the nucleus, respectively. β-catenin was detected using primary beta-catenin and TRITC-conjugated secondary antibodies (orange). As shown, PF cells consistently displayed readily detectable peri-nuclear staining as well as diffuse staining throughout the cytoplasm after three days on collagen culture. In contrast, DD cells displayed distinct β-catenin staining at the periphery of some cells adjacent to the cell membrane, with little or no β-catenin evident in the cytoplasm after three days on collagen culture.