Figure 2

Type 1 collagen culture effects on β-catenin accumulation are consistent between patient samples. A Representative western immunoblot analysis of additional patient matched sets of PF cells (PF2 and PF3) and DD cells (PD2 and PD3) cultured for 72 hours on either tissue culture plastic (Plc) or type 1 collagen (Col) and probed for β-catenin. β-actin levels were assessed to confirm equal total protein loading. As indicated, (arrows) a decrease in β-catenin levels is evident in DD cells cultured on collagen for 72 hours. B Pooled densitometric analysis of 6 patient matched PF and DD cell samples cultured for 72 hours on type 1 collagen normalized to β-actin. As shown, a consistent, significant decrease in band density indicating decreased β-catenin levels is evident in DD cells cultured on type 1 collagen coated dishes for 72 hours relative to 0 hour cultures (*, p < 0.05).