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Table 1 Effect of 2ME2 on Quantitative Histomorphometry Parameters

From: Disease modifying and antiangiogenic activity of 2-Methoxyestradiol in a murine model of rheumatoid arthritis

Treatment Group

Total Osteoclast Numbers

Articular cartilage area without proteoglycan staining (%)

Damaged articular cartilage surface (%)

Thickness of total articular cartilage area (μM)

Vehicle Control

33.9

17.23

47.3

26.06

2ME2 100 mg/kg

0

0.18*

0*

32.64*

2ME2 10 mg/kg

5.7*

6.71

27.9

27.34

2ME2 1 mg/kg

21.9*

11.21

37.0

27.5

  1. Quantitative histomorphometry was performed using Ostemeasure software interfaced with a Nikon Eclipse E400 light/epiflourescent microscope and video system. Histomorphometric measurements of the articular area and surface were performed using Safranin O stained slides and osteoclast number was counted using acid phosphatase/toluidine blue stained slides using blinded samples. The area of interest included the area from the talus-navicular joint to the navicular to the second metatarsal joint. Results are presented as mean determinations from 10 observations (*p < 0.05 compared to vehicle control).
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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