Western blot results. Western blots were used to determine Gli3 protein abundance in nuclear extracts from the hindlimb tissue of ICTEV model rat and normal control rat embryos. Compared to the normal control, Gli3 expression was significantly enhanced in model rats. With birth drawing near, Gli3 protein expression in both model and normal rats had a tendency to decrease. Lane 1 is the protein of ICTEV model rat embryos at GD15, lane 2 is the protein of normal rat embryos at GD15, lane 3 is the protein of ICTEV model rat embryos at GD17 and lane 4 is the protein of normal rat embryos at GD17.