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Morphological, histochemical, and interstitial pressure changes in the tibialis anterior muscle before and after aortofemoral bypass in patients with peripheral arterial occlusive disease

  • Maria Albani1Email author,
  • Angelos Megalopoulos2,
  • Dimitris Kiskinis2,
  • Sotirios A Parashos4,
  • Nikolaos Grigoriadis3 and
  • Olympia Guiba-Tziampiri1
BMC Musculoskeletal Disorders20023:8

DOI: 10.1186/1471-2474-3-8

Received: 11 October 2001

Accepted: 25 February 2002

Published: 25 February 2002

Abstract

Background

Morphological and electrophysiological studies of ischemic muscles in peripheral arterial disease disclosed evidence of denervation and fibre atrophy. The purpose of the present study is to describe morphological changes in ischemic muscles before and after reperfusion surgery in patients with peripheral occlusive arterial disease, and to provide an insight into the effect of reperfusion on the histochemistry of the reperfused muscle.

Methods

Muscle biopsies were obtained from the tibialis anterior of 9 patients with chronic peripheral arterial occlusive disease of the lower extremities, before and after aortofemoral bypass, in order to evaluate the extent and type of muscle fibre changes during ischemia and after revascularization. Fibre type content and muscle fibre areas were quantified using standard histological and histochemical methods and morphometric analysis. Each patient underwent concentric needle electromyography, nerve conduction velocity studies, and interstitial pressure measurements.

Results

Preoperatively all patients showed muscle fibre atrophy of both types, type II fibre area being more affected. The mean fibre cross sectional area of type I was 3,745 μm2 and of type II 4,654 μm2 . Fibre-type grouping, great variation in fibre size and angular fibres were indicative of chronic dennervation-reinnervation, in the absence of any clinical evidence of a neuropathic process. Seven days after the reperfusion the areas of both fibre types were even more reduced, being 3,086 μm2 for type I and 4,009 μm2 for type II, the proportion of type I fibres, and the interstitial pressure of tibialis anterior were increased.

Conclusions

The findings suggest that chronic ischemia of the leg muscles causes compensatory histochemical changes in muscle fibres resulting from muscle hypoxia, and chronic dennervation-reinnervation changes, resulting possibly from ischemic neuropathy. Reperfusion seems to bring the oxidative capacity of the previously ischemic muscle closer to normal.

Background

Structural and functional adaptations take place in working and resting muscles when the blood flow is insufficient to satisfy energy requirements [13]. Partial ischemia for short periods of time may cause cell damage in humans [4, 5], while metabolic changes in leg muscles have been reported after 16 hours of ischemia [6]. Morphological and electrophysiologic studies of ischemic muscles in peripheral occlusive arterial disease disclosed evidence of denervation and type II fibre atrophy [79]. Several studies have demonstrated an increase in the oxidative capacity of ischemic muscles in patients with atherosclerotic peripheral occlusive arterial disease [8, 1012] while other authors reported reduced oxidative capacity in leg muscles of patients with intermittent claudication [13, 14].

The crucial role of increased blood flow in striated muscles after an ischemic period has been studied extensively in the heart muscle. Recent publications [15, 16] have shown biochemical, morphological and ultra structural changes in the heart muscle after reperfusion in experimentally induced prolonged global ischemia or after the use of free oxygen radical inhibitors.

Although biochemical changes occurring in other organs during reperfusion following ischemia have been described in numerous studies [1721], there is hardly any information about changes in human muscle fibres during reperfusion after bypass operations. The purpose of the present study is to identify the histochemical profile of ischemic skeletal muscles before and after the revascularization operation in patients with peripheral occlusive arterial disease.

Materials and Methods

Subjects

Nine 64 ± 12 year-old men with atherosclerotic lesions at the aortoiliac level (Fontaine stage II) were investigated. None of them showed clinical signs of peripheral neuropathy, and any history of diabetes mellitus. All patients were submitted to a reconstructive surgical procedure, which consisted of uni- or bilateral aortofemoral bypass grafting, and were given 100 mg acetylsalicylic acid per day as postoperative treatment. The patients were informed on the nature of the study, with the possible risks of the procedures, and they gave consent prior to inclusion in the study. The Human Subjects Committee of the Aristotle University of Thessaloniki approved the experimental protocol.

Procedures

Electrophysiologic studies

All patients underwent standard electromyographic examination of both lower extremities, before the first biopsy to exclude peripheral neuropathy. Motor nerve conduction velocities with corresponding F-waves of the posterior tibial and peroneal nerves and sensory nerve conduction velocities of the sural nerves were obtained bilaterally, using standard technique, and a Neuropack 2® (Nihon Kohden). Concentric needle electromyographic examination was limited to the tibialis anterior, extensor digitorum brevis and abductor hallucis brevis bilaterally. None of the patients showed electrophysiologic evidence of a peripheral neuropathy.

Muscle Biopsy

Two tissue samples were obtained from each patient during each biopsy under local anaesthesia (1 % lidocaine), from the tibialis anterior muscle. The samples were dissected out in blocks of 5 × 5 × 5 mm3, were taken from a well-defined portion of the muscle belly, to a depth of about 2 cm beneath the fascia, at the half distance between the ankle and the knee. The first biopsy was taken during the reconstructive surgery procedure and the second biopsy 7 days after the operation. Each sample was then frozen in melting isopentane (-60°C) cooled in liquid nitrogen, and stored in a freezer (-80°C).

Intramuscular pressure measurement

Muscle compartmental pressure was measured pre- and postoperatively, at the 1st & 7th postoperative day, by inserting a special sterile 18 gauge side ported needle 6.4 cm long, in the tibialis anterior muscle, which was connected to the Stryker intra-compartmental pressure monitor system (Stryker, 295–1 Pressure), a hand held monitor system specifically designed for measuring tissue fluid pressures.

Histology and Enzyme histochemistry

Transverse serial sections of 9 μm – thick of each sample were cut in a cryostat microtome at -20°C. The sections were processed with eosin-hematoxylin, hematoxylin-van Gieson for routine histologic examination; with succinic dehydrogenase (SDH) and NADH-trichrome reductase [22] for oxidative enzyme activity [21]; with myosin-ATPase for fibre typing [22]; and with periodic acid-Schiff ((PAS) for glycogen [22].

Morphometry

Morphometric parameters were determined by projecting the microscope slides (Zeiss) via a camera lucida onto a calibrated grid (Huston Instrument TG 1011 digitizer) linked to a desktop computer. A program was developed in GW-BASIC for digitation and further calculations. Fibre-type cross-sectional area and fibre type proportion were determined by counting 200 contiguous fibres of the central part of each tissue sample.

Statistical methods

Continuous data were reported as mean value ± standard deviation and categorical data as percentages. Paired Student's test was used for comparing differences between the mean values before and after reperfusion, and chi-square was used to compare the differences between the fibre-type proportions before and after the reperfusion. Differences were considered significant at P values < 0.05.

Results

Histological examination

First biopsy (before bypass grafting)

The routine histologic examination revealed pericellular leucocyte infiltration between and within degenerating fibre, and necrotic muscle fibres (Fig. 1a and 1b), regenerated muscle fibres, and fibre size diversity (Fig. 1b and 1c). All patients showed muscle fibre atrophy, which was more pronounced in type II fibres, and great fibre size diversity; the mean area of type II fibres was 4,654 ± 2,626 μm2, a 18–43% reduction when compared to previous reports on healthy subjects of similar age (Table 1). Type I fibre size was 3,745 ± 2,094 μm2, a value which is also 7.5–31% less than the reported value for healthy subjects of similar age (Table 1). The mean proportion of type I fibres was 58.3 % and of type II was 41.7%. NADH-tetrazolium reductase staining demonstrated 55.6 % of oxidative fibres and 44.4 % of nonoxidative (Table 2). Abnormalities typical for denervation and reinnervation, i.e., small angular fibres (Fig. 1b and 1c) and small areas of fibre-type grouping (Fig. 1d) were seen in all samples.
Figure 1

Micrographs from sections of tibialis anterior muscle from patients with peripheral occlusive disease before reperfusion procedure (calibration: 100 μm). (a) Eosin-hematoxylin. (b) SDH; phagocytosis and necrotic muscle fibres (arrows). (c) Myosin-ATPase, acid preincubation; small groups of atrophic angular muscle fibres (arrows), and fibre size diversity. (d) NADH; small areas of fibre-type grouping.

Table 1

Fibre type areas, proportions and age of subjects as reported by other authors

Authors

Type I area (μm2)

Type I (%)

Type II area (μm2)

Type II (%)

Age and sex (yrs – M/F)

Jakobsson F et al [22]

4,050 ± 8,900

84 ± 9

5,700 ± 1970

15 ± 8

70, M&F

Angquist et al [23]

5,407.86

74

7,386.06

26

59, M 47, F

Friden et al [24]

5,430 ± 883

69

8,132 ± 1747

31

26, M

Henriksson-Larsen et al [13]

 

70

 

30

 

Sjøstrøm et al [8]

5,024

70

7,234.56

30

59, M 47, F

values are means ± sd

Table 2

Muscle fibre cross-sectional areas and fibre type proportions of tibialis anterior biopsy samples before and after the reconstructive surgical procedure (values are means ± sd)

 

Before

After

Significance

OXIDATIVE FIBRES

   

Area (μm2)

3546.8 ± 1413

3264.8 ± 769

P < 0.001**

Fibre proportion

55.7%

62.5%

NS**

NON OXIDATIVE FIBRES

   

Area (μm2)

4003.5 ± 1388

3771.3 ± 1238

P < 0.05**

Fibre proportion

44%

37.5%

NS**

* Student's t-test ** Chi-square NS: not statistically significant

Second Biopsy (7 days after bypass grafting)

Seven days after the aortofemoral bypass grafting, the main morphological muscle fibre characteristics remained unchanged (Fig. 2). Muscle fibre atrophy was again observed (Fig. 2a and 2b; Tables 2, 3). PAS staining did not show any obvious differences in muscle fibres before and after the reperfusion (data not shown). The mean fibre cross-sectional area, reduced for both fibre types, was 4,008 ± 1917 μm2 for type II, and 3,085 ± 1340 am for type I fibres. The high standard deviation values of all area measurements both before and after the repair surgery procedure suggests a great variation of fibre size, which reflects the presence of many atrophic and regenerating muscle fibres among the normal ones. The proportion of type II fibres was decreased to 33.8% while that of type I fibres was increased to 66.2%, although not statistically significant. Abnormalities typical for denervation and reinnervation were again seen in all samples (Fig. 2).
Figure 2

Micrographs from sections of tibialis anterior muscle from patients with peripheral occlusive disease after reperfusion procedure (calibration: 70 μm). (a) Myosin-ATPase, acid preincubation, (b) NADH; small angular atrophic muscle fibres of both types in small groups or dispersed (arrows). (c) NADH. (d) ATPase; fibre-type grouping.

Table 3

Morphometric data of tibialis anterior muscle fibres (mean ± SD), in the muscle biopsy samples before and after reperfusion.

 

Before

After

Significance

TYPE I FIBRES

   

Area (μm2)

3,745 ± 2,094

3,086 ± 1,340

P < 0.001**

Fibre proportion

58.2%

66.2%

NS**

TYPE II FIBRES

   

Area (μm2)

4,654.5 ± 2626

4,009 ± 1,917

P < 0.001**

Fibre proportion

41.7%

33.8%

NS**

* Student's t-test ** Chi-square NS: not statistically significant

Interstitial pressure measurements

The interstitial pressure was 6.5 ± 3.3 mm Hg in the tibialis anterior of ischemic limbs before the reconstructive surgery procedure, but was increased to 10.30 ± 5 mm Hg on the 1st postoperative day, and to 14.38 ± 9.8 mm Hg on the 7th postoperative day. Two-tailed paired t-test showed difference only between the preoperative and postoperative values for P < 0.05; no statistically significant difference was found between the 1st and the 7th postoperative day values.

Discussion

The main morphological characteristics of ischemic muscles in peripheral occlusive arterial disease patients with peripheral occlusive arterial disease observed in the present study are similar to those reported by Sjøstrøm et al [8]. The presence of muscle fibre grouping in all biopsies suggests a process of chronic dennervation-reinnervation, a possibility previously disputed. The question of whether fibre-type grouping and nerve-ending degeneration might be age-related rather than the result of ischemia is still open to debate. The effect of ischemia and reperfusion on motor nerves has been described in humans [9, 27] and in experimental animal models [2832], nevertheless the underlying mechanism is incompletely understood. During reperfusion of the acutely ischemic tibialis anterior of rabbits, the deep peroneal nerve's conduction was impaired and the action potential was abolished [29]. Tourniquet ischemia for 20 min prevents paw plantar flexion following stimulation of the tibial nerve [31], and causes changes in sympathetic or parasympathetic activity, when applied for longer periods [30]. The electromyographic findings of the present study, i.e. normal conduction velocities with evidence of chronic dennervation-reinnervation are in agreement with previous reports in patients with peripheral occlusive arterial disease [9], and would be consistent with the notion of a parallel neuropathic process, likely ischemic in etiology [27]. The fact that no overt neuropathy was apparent in our patients, either on clinical examination, or on nerve conduction testing, is most likely the result of the relatively early peripheral occlusive arterial disease stage of our patients.

Groups of small regenerated muscle fibres were noted in this study on light microscope both before and after 7 days reperfusion with no change in appearance, whereas Karpati et al [33] on electron microscope after 7 days of experimentally induced ischemia on Sprague Dawley rats observed mitochondrial debris and large membrane-lined space. The same authors reported no change in the appearance of regenerating fibres found in ischemic muscle for up to 3 months. In this study the presence of regenerated and atrophic muscle fibres is also illustrated by the heterogeneity found within both fibre populations. Similar findings were reported by Sjøstrøm [8] who suggested that these changes were due either to loss of large efferent nerves, and possibly of anterior horn cells, or to the cumulative effect of incidental diseases.

Ischemia affects each muscle fibre type in different ways according to its particular metabolic and functional properties. The type I predominance found in our study, as well as the muscle fibre areas of tibialis anterior muscle are in accordance with the findings of other authors of age-matched human subjects [24, 34, 35]. However, as a result of conflicting reports regarding muscle oxidative capacity in patients with peripheral occlusive arterial disease [13, 8, 10, 11, 13, 14, 21, 23, 34] the relationship between local pathology and clinical symptomatology remains elusive. Jennische [36] found a selective vulnerability of the fast glycolytic fibres to experimental ischemia in rats. By contrast, slow-oxidative fibres were spared. The author attributed the difference to the higher content of free-radical scavenger compounds in the more tolerant fibres, and to their different capability for calcium uptake. Other investigators reported an increase in mitochondrial volume in chronically ischemic muscles [25, 32], while, in an apparent contradiction, decreased muscle oxidative capacity has been reported in patients with intermittent claudication [13], and decreased dehydrogenase activity following 6 hours of ischemia in dogs [37].

A possible explanation to this discrepancy was given by Clyne et al [38], who stated that during the initial stages of ischemia skeletal muscle tries to compensate for the oxygen deficiency, while at later stages as blood flow is further decreased and functional demands are increased, it looses the functional adaptations.

Conclusions

The morphometric, histological and histochemical observations of our study suggest that type I fibres are at least somewhat vulnerable to ischemia, although not to the same degree as type II fibres. The proportion of type I fibres of the ischemic tibialis anterior increased to 66.2% after reperfusion (Table 3), a number closer to that reported for normal subjects [8, 13, 2224], whereas the proportion of type II fibres proportionally decreased (Table 3), eventhough these changes in proportions of fibre types were not statistically significant. The mean cross-sectional area of both muscle fibre types was decreased after reperfusion, 17.6% for type I and 13.8% for type II, conceivably as a result of a postbypass efflux of intracellular compounds to the interstitial space and a subsequently increased interstitial fluid volume. Such statistically significant increase in interstitial fluid volume would also explain the observed postoperative increase in the interstitial fluid pressure.

Our findings indicate that in patients with peripheral occlusive arterial disease at the aortoiliac level, both muscles and nerves are affected. Chronic dennervation-reinervation mechanisms may account for the fibre type grouping, while reperfusion may favourably affect the oxidative properties of chronically ischemic muscles.
Figure 3

Micrographs from serial adjacent section of the same field (each muscle fibre stained differently in a and b is marked with a number) of rectus abdominis muscle (used as control) before the reconstructive surgery (calibration 150 μm). (a) Myosin-ATPase (acid preincubation), (b) NADH; normal appearance.

Declarations

Acknowledgements

The authors thank Dora Kapoucranidou for her technical assistance. We also thank Professor Olga Hudlicka for her comments on the manuscript.

Authors’ Affiliations

(1)
Department of Physiology, Medical School, Aristotle University of Thessaloniki
(2)
Dept of Surgery, Medical School, Aristotle University of Thessaloniki
(3)
Dept of Neurology, Medical School, Aristotle University of Thessaloniki
(4)
Minneapolis Clinic of Neurology

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  39. Pre-publication history

    1. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/3/8/prepub

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