A single-nucleotide polymorphism rs708567 in the IL-17RC gene is associated with a susceptibility to and the curve severity of adolescent idiopathic scoliosis in a Chinese Han population: a case-control study
© Zhou et al.; licensee BioMed Central Ltd. 2012
Received: 10 March 2012
Accepted: 14 September 2012
Published: 21 September 2012
Although the pathogenesis of adolescent idiopathic scoliosis (AIS) remains controversial, genetic factors are thought to play key roles in the development of AIS. In a recent genome-wide association study, a polymorphism in the interleukin-17 receptor C (IL-17RC) gene was reported to be associated with the susceptibility to AIS, implicating IL-17RC as a novel predisposing gene for AIS. However, as this association has not been replicated in other populations, its global applicability remains unclear.
A total of 529 Chinese girls with AIS and 512 healthy age-matched controls were recruited in this case–control study from June 2007 to December 2009. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was performed to detect the genotype of the single-nucleotide polymorphism (SNP) rs708567 in the IL-17RC gene. Case–control and case-only studies were performed to determine the association between the IL-17RC gene polymorphism and the susceptibility to and curve severity of AIS.
The GG genotype and G allele frequencies were significantly higher in the AIS patients than in the controls (χ 2 test: P = 0.023 and 0.028, respectively). The risk for the GG genotype is 1.550-fold (95% CI: 1.062 - 2.261) higher than the AG genotype, and the risk for the G allele is 1.507-fold (95% CI: 1.046 - 2.172) higher than the A allele. Additionally, a subgroup of skeletally mature AIS patients (n = 241) who carried the GG genotype showed a significantly higher mean maximum Cobb angle than those carrying the AG genotype (36.01 ± 13.12° vs. 28.92 ± 7.43°, P = 0.007).
This study confirms the significant association between the IL-17RC gene polymorphism and the susceptibility to and curve severity of AIS in a Chinese Han population, suggesting that the IL-17RC gene is an AIS-predisposing gene in Chinese Han population.
KeywordsAdolescent idiopathic scoliosis IL-17RC Single-nucleotide polymorphism
Adolescent idiopathic scoliosis (AIS) is one of the most common spinal deformities, with a world-wide pediatric prevalence of 2-3% . In recent decades, many etiological factors have been proposed for AIS, such as uncoupled growth of the spinal column , paraspinal muscle dysfunction[3, 4], hormonal disturbances [5–7], and inflammation. However, no single factor can explain all of the clinical characteristics of AIS. Therefore, AIS is generally regarded as a multi-factorial disorder for which hereditary or genetic factors likely play important roles.
The genetic factors contributing to AIS have been described in many studies. Genome-wide linkage analysis studies have identified several variations in chromosomal regions, including 9q31.2–q34.2, 12p, 17p11 and so on [9–14], as being associated with an increased susceptibility to AIS. Through genetic association studies, several genes, such as tryptophan hydroxylase 1 (TPH1) , estrogen receptor α (ESR1) , and the matrilin-1 gene (MATN1) , have been identified as genes that predispose an individual to AIS. Furthermore, a number of genes have also been found to be related to the curve severity of AIS[18, 19].
In a recent genome-wide association study using data from 137 AIS patients of European ancestry, Dormans JP et al. reported that SNP rs708567 (a common missense mutation) was associated with susceptibility to AIS. SNP rs708567 is located on chromosome 3p25.3 and occurs in an exon of the IL-17 receptor C (IL-17RC) gene, which codes for an integral member of the IL-17R complex . As an essential part of the IL-17 cytokine/IL-17R signaling axis, the IL-17R complex mediates the signal transduction of the IL-17 cytokine family, which promotes the production of pro-inflammatory cytokines, such as tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) . The findings of Dormans JP et al.  identified IL-17RC as a novel predisposing gene for AIS, thus providing significant information regarding the genetic etiology of AIS.
However, due to the small sample sizes, population stratification, variability in the control population, and racial and ethnic differences, genetic association studies may produce spurious associations . Therefore, the purpose of this case–control study was to replicate the previously reported association between the IL-17RC gene polymorphism and the susceptibility to AIS and to further investigate the contribution of this polymorphism to the curve severity of AIS within a Chinese Han population. Our results suggest that the IL-17RC gene is an AIS-predisposing gene in Chinese Han population.
This study included two sections: a case–control study and a case-only study.
The case–control study evaluated the genetic association between the predisposition to AIS and the rs708567 SNP within the IL-17RC gene. The case-only study evaluated the genetic association between rs708567 and curve severity in a subgroup of AIS patients who had reached skeletal maturity. Skeletal maturity was defined as being at least 16 years old and having a Risser sign of 5 (the Risser sign is a grading system for the ossification of the iliac apophysis). As treatments may change the natural history of AIS, patients who had received any previous treatment were initially excluded from the case-only study.
A total of 698 Chinese girls with AIS, aged 11 to 18 years, and 512 gender- and age-matched controls were consecutively recruited at our scoliosis clinic from June 2007 to December 2009. A diagnosis of AIS was made according to a clinical examination and standard standing posteroanterior whole-spine radiography. The scoliosis curve severity was measured according to the Cobb method, and patients (529 cases) with a major Cobb angle ranging from 20° to 100° were included in the study. Patients (104 cases) with a curve less than 20° were excluded because of a possible “false-positive” diagnosis of AIS, and patients (65 cases) with a curve greater than 100° were also excluded because of a possible association with intraspinal and brain stem abnormalities. In addition, patients who were diagnosed with congenital scoliosis, neuromuscular diseases, endocrine diseases, skeletal dysplasia, connective tissue abnormalities or scoliosis secondary to some other disorder were excluded from the subset. The healthy controls were recruited during a regular school health-screening program. To rule out any spinal deformity, the Adam forward-bending test  was performed by an experienced orthopedic surgeon for all of the controls. In addition, no subjects showed evidence of bone disease or growth disturbances, systemic illness or other conditions known to affect bone metabolism or had a history of recent steroid intake.
The study protocols were approved by the University and Hospital Ethics Committee. Informed consent was obtained from all of the subjects and their parents prior to the DNA analysis.
Blood sampling and genotyping
As described by Dormans JP et al. , SNP rs708567 in the IL-17RC gene is associated with a susceptibility to AIS. The present study focused only on this SNP, and rs708567 genotyping was performed using PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism) in the Chinese Han population described above.
Primers and restriction enzymes for PCR-RFLP analysis
Annealing Temperature (°C)
PCR Product Size (bp)
Fragment Size After Enzymatic Cleavage (bp)
Forward Primer: 5’-AGTAGGGTAGGCCTGGAAGG-3’
Reverse Primer: 5’-CACTGGGAAGAGCCTGAAGA-3’
AG: 161 + 49
A power analysis was performed using PASS (version 11; NCSS, Kaysville, UT; http://www.ncss.com/pass.html). The other statistical analyses were performed using SPSS 13.0 for Windows (SPSS Inc., Chicago, IL). Hardy-Weinberg equilibrium (HWE) was tested by a goodness-of-fit χ 2 test. The case–control difference for the allele and genotype frequencies was analyzed using the χ 2 test. The odds ratios (ORs) and 95% confidence interval (95% CI) ranges were calculated. A one-way analysis of variance was used to compare the mean maximum Cobb angle of the different genotypes in the AIS patients. A P-value lower than 0.05 was considered to be statistically significant.
A total of 529 Chinese AIS girls with a mean age of 14.54 ± 1.62 years and 512 gender- and age-matched controls with a mean age of 14.36 ± 1.93 years were recruited for this study. The mean Risser sign of the AIS patients and the mean maximum Cobb angle were 3.17 ± 1.89 (range 0–5) and 38.30 ± 16.71° (range 20° - 100°), respectively. Our sample size had an 81% power to detect the AIS-relevant SNP within the IL-17RC gene. The genotype frequencies showed no significant deviation from Hardy-Weinberg equilibrium (HWE) in either the AIS group or control group. Of these 529 AIS patients, 241 patients (mean maximum Cobb angle of 35.24 ± 12.81°) who had reached skeletal maturity were included in the case-only study.
Association of SNP rs708567 with the susceptibility to AIS
Distribution of the genotype and allele frequencies of SNP rs708567 in the IL-17RC gene in AIS patients (n = 529) and controls (n = 512)
Group, n (%)
OR (95% Confidence Interval)
1.550 (1.062 - 2.261)
0.645 (0.442 - 0.941)
1.507 (1.046 - 2.172)
0.664 (0.460 - 0.956)
Association of SNP rs708567 with curve severity in AIS
Association of the IL-17RC gene polymorphism with curve severity in AIS patients who had reached skeletal maturity
Number of Subjects
Mean Maximum Cobb Angle (Mean ± SD)
36.01 ± 13.12
28.92 ± 7.43
Previous population studies have reported a significantly higher AIS incidence of 15.8% in first-degree relatives of AIS patients, 36% in dizygotic twins, and 73% in monozygotic twins [25, 26]. In addition, a significantly higher incidence of AIS was found in females than males, with a female-to-male ratio of approximately 10:1. Given the documented higher incidence of AIS within families and the sexual dimorphism of AIS occurrence, genetics may offer the best possibility to unravel the etiology of AIS.
A genetic association study is one of the most effective methods for identifying and characterizing the genomic variants that underlie the susceptibility to a multifactorial disease . However, the quality of genetic association may be affected by many factors, such as a small sample size, population stratification, and racial and ethnic differences, and false-positive associations may be produced. For example, Zhang et al.  initially reported that SNP rs1256120 in the estrogen receptor β gene (ESR2) was associated with a higher susceptibility to and the curve severity of AIS in Chinese females using data from 176 AIS patients and 80 controls. However, Takahashi et al.  could not replicate this association in a Japanese population using data from 798 AIS patients and 637 controls. Therefore, Neale et al.  advocated that any positive association findings in gene-based studies should be confirmed in a different population using a larger sample size.
In a recent genetic association study, Dormans JP et al.  reported that SNP rs708567 (a common missense mutation) in the IL-17RC gene is associated with a higher susceptibility to AIS in a population of European ancestry, suggesting that IL-17RC may be an AIS susceptibility gene. However, in a subsequently published association study using data from 419 American AIS patients and their families, Sharma S et al.  did not find the association between the IL-17RC gene polymorphism and the susceptibility to AIS. Furthermore, Takahashi Y et al.  also did not report the association between this SNP and an AIS predisposition in Japanese female adolescents in another genome-wide association study. Therefore, it is necessary to perform an additional study to confirm the results for SNP rs708567.
In the present study, we re-evaluated the association between SNP rs705867 and the predisposition to AIS using data from a Chinese Han population. Our results show that both the GG genotype and G allele have a significantly higher frequency in AIS patients than the controls (P = 0.023 and P = 0.028, respectively). Our results are consistent with the finding of Dormans JP et al. , confirming that IL-17RC is a novel predisposition gene for AIS. The discrepancy between these results may reflect the issue of heterogeneity to detect modest effect sizes. In addition, our results further revealed that AIS patients with the GG genotype have a higher mean maximum Cobb angle than those with the AG genotype, indicating that the IL-17RC gene polymorphism is also associated with the curve severity in AIS patients.
The human IL-17RC gene on chromosome 3 contains 19 exons and spans 16,550 bp within the chromosomal region 3p25.3 to 3p24.1. The IL-17RC gene encodes a single-pass type I transmembrane protein (IL-17 receptor C, IL-17RC), which was characterized as an integral part of the IL-17 receptor (IL-17R) complex . The IL-17R complex mediates IL-17 effector function through the IL-17 cytokine/IL-17R complex signaling axis, and current evidence indicates that both the dysfunction of IL-17RC and the IL-17 cytokine/IL-17R signaling axis are implicated in a number of human diseases. As expected, given its central role in regulating inflammation, dysfunctions in the IL-17 cytokine/IL-17R signaling axis can promote the production of several pro-inflammatory cytokines, such as tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), and increase the activity of metalloproteinases (MMPs), which play important roles in disc degeneration . Furthermore, recent studies have also shown that an impaired IL-17 cytokine/IL-17R signaling axis drives the pathogenesis of bone destruction, in part by inducing the expression of RANKL in osteoblasts [34–36]. Thus, regarding the role of IL-17RC and/or the IL-17 cytokine/IL-17R signaling axis in disc and bone destruction, the rs708567 polymorphism may impair the function of the IL-17RC gene and accelerate disc degeneration and bone destruction, which may lead to the occurrence and progression of scoliosis. Indeed, defects within the discs and bones of AIS patients have been widely described in previous studies [37, 38].
Overall, we found a positive association between SNP rs708567 and the susceptibility to and curve severity of AIS in a Chinese Han population. Unfortunately, the specific influence of SNP rs708567 on IL-17RC gene function remains unknown. As described by Dormans JP et al., SNP rs708567 is a common missense mutation (S111 L) within the IL-17RC gene, suggesting that synergistic interactions between SNP rs708567 and other polymorphisms may play an important role in impairing IL-17RC gene function. Therefore, further investigations will be performed using the method of pair-wise linkage disequilibrium (LD) analysis in our next study to clarify the possible synergistic interactions between SNP rs708567 and other polymorphisms and their effect on the susceptibility to AIS. In addition, a potential limitation that should not be ignored is the false-negative result of the Adam forward-bending test, which may lead to an ascertainment bias with regard to the controls. Therefore, further investigations using other samples are necessary to confirm this positive association.
This study identified a significant association between an IL-17RC gene polymorphism and the susceptibility to and curve severity of AIS in a Chinese Han population, indicating that IL-17RC is a predisposing gene for AIS with a single thoracic curve. However, further studies are necessary to confirm these positive findings in other ethnicities.
We gratefully recognize the financial support of the Nanjing Medical Science and technique Development Foundation and the National Natural Science Foundation of China (NO. 81171767 and NO. 30901570).
- Sharma S, Gao X, Londono D, Devroy SE, Mauldin KN, Frankel JT, Brandon JM, Zhang D, Li QZ, Dobbs MB, Gurnett CA, Grant SF, Hakonarson H, Dormans JP, Herring JA, Gordon D, Wise CA: Genome-wide association studies of adolescent idiopathic scoliosis suggest candidate susceptibility genes. Hum Mol Genet. 2011, 20: 1456-1466. 10.1093/hmg/ddq571.View ArticlePubMedPubMed CentralGoogle Scholar
- Guo X, Chau WW, Chan YL, Cheng JC: Relative anterior spinal overgrowth in adolescent idiopathic scoliosis Results of disproportionate endochondral membranous bone growth. J Bone Joint Surg Br. 2003, 85: 1026-1031. 10.1302/0301-620X.85B7.14046.View ArticlePubMedGoogle Scholar
- Spencer GS, Eccles MJ: The proportion and size of type 1 and type 2 skeletal muscle fibres measured using a computer-controlled microscope. J Neurol Sci. 1976, 30: 143-154. 10.1016/0022-510X(76)90262-8.View ArticlePubMedGoogle Scholar
- Dastych M, Vlach O: Zinc status in patients with idiopathic scoliosis. Spine (Phila Pa 1976). 1990, 15: 65-66. 10.1097/00007632-199002000-00002.View ArticleGoogle Scholar
- Machida M, Saito M, Dubousset J, Yamada T, Kimura J, Shibasaki K: Pathological mechanism of idiopathic scoliosis: experimental scoliosis in pinealectomized rats. Eur Spine J. 2005, 14: 843-848. 10.1007/s00586-004-0806-1.View ArticlePubMedGoogle Scholar
- Machida M, Dubousset J, Yamada T, Kimura J, Saito M, Shiraishi T, Yamagishi M: Experimental scoliosis in melatonin-deficient C57BL/6J mice without pinealectomy. J Pineal Res. 2006, 41: 1-7. 10.1111/j.1600-079X.2005.00312.x.View ArticlePubMedGoogle Scholar
- Moreau A, Wang DS, Forget S, Azeddine B, Angeloni D, Fraschini F, Labelle H, Poitras B, Rivard CH, Grimard G: Melatonin signaling dysfunction in adolescent idiopathic scoliosis. Spine (Phila Pa 1976). 2004, 29: 1772-1781. 10.1097/01.BRS.0000134567.52303.1A.View ArticleGoogle Scholar
- Aulisa L, Papaleo P, Pola E, Angelini F, Aulisa AG, Tamburrelli FC, Pola P, Logroscino CA: Association between IL-6 and MMP-3 gene polymorphisms and adolescent idiopathic scoliosis a case–control study. Spine (Phila Pa 1976). 2007, 32: 2700-2702. 10.1097/BRS.0b013e31815a5943.View ArticleGoogle Scholar
- Ocaka L, Zhao C, Reed JA, Ebenezer ND, Brice G, Morley T, Mehta M, O'Dowd J, Weber JL, Hardcastle AJ, Child AH: Assignment of two loci for autosomal dominant adolescent idiopathic scoliosis (AIS) to chromosomes 9q31.2-q34.2 and 17q25.3-qtel. J Med Genet. 2008, 45: 87-92.View ArticlePubMedGoogle Scholar
- Alden KJ, Marosy B, Nzegwu N, Justice CM, Wilson AF, Miller NH: Idiopathic scoliosis: identification of candidate regions on chromosome 19p13. Spine (Phila Pa 1976). 2006, 31: 1815-1819. 10.1097/01.brs.0000227264.23603.dc.View ArticleGoogle Scholar
- Raggio CL, Giampietro PF, Dobrin S, Zhao C, Dorshorst D, Ghebranious N, Weber JL, Blank RD: A novel locus for adolescent idiopathic scoliosis on chromosome12p. J Orthop Res. 2009, 27: 1366-1372. 10.1002/jor.20885.View ArticlePubMedPubMed CentralGoogle Scholar
- Gurnett CA, Alaee F, Bowcock A, Kruse L, Lenke LG, Bridwell KH, Kuklo T, Luhmann SJ, Dobbs MB: Genetic linkage localizes an adolescent idiopathic scoliosis and pectus excavatum gene to chromosome 18q. Spine (Phila Pa 1976). 2009, 34: E94-E100. 10.1097/BRS.0b013e31818b88a5.View ArticleGoogle Scholar
- Salehi LB, Mangino M, De Serio S, De Cicco D, Capon F, Semprini S, Pizzuti A, Novelli G, Dallapiccola B: Assignment of a locus for autosomal dominant idiopathic scoliosis (IS) to human chromosome 17p11. Hum Genet. 2002, 111 (4–5): 401-404.View ArticlePubMedGoogle Scholar
- Edery P, Margaritte-Jeannin P, Biot B, Labalme A, Bernard JC, Chastang J, Kassai B, Plais MH, Moldovan F, Clerget-Darpoux F: New disease gene location and high genetic heterogeneity in idiopathic scoliosis. Eur J Hum Genet. 2011, 19: 865-869. 10.1038/ejhg.2011.31.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang H, Wu Z, Zhuang Q, Fei Q, Zhang J, Liu Y, Wang Y, Ding Y, Qiu G: Association study of tryptophan hydroxylase 1 and arylalkylamine N-acetyltrans-ferase polymorphisms with adolescent idiopathic scoliosis in Han Chinese. Spine (Phila Pa 1976). 2008, 33: 2199-2203. 10.1097/BRS.0b013e31817c03f9.View ArticleGoogle Scholar
- Wu J, Qiu Y, Zhang L, Sun Q, Qiu X, He Y: Association of estrogen receptor gene polymorphisms with susceptibility to adolescent idiopathic scoliosis. Spine (Phila Pa 1976). 2006, 31: 1131-1136. 10.1097/01.brs.0000216603.91330.6f.View ArticleGoogle Scholar
- Qiu XS, Tang NL, Yeung HY, Lee KM, Hung VW, Ng BK, Ma SL, Kwok RH, Qin L, Qiu Y, Cheng JC: Melatonin receptor 1B (MTNR1B) gene polymorphism is associated with the occurrence of adolescent idiopathic scoliosis. Spine (Phila Pa 1976). 2007, 32: 1748-1753. 10.1097/BRS.0b013e3180b9f0ff.View ArticleGoogle Scholar
- Inoue M, Minami S, Nakata Y, Kitahara H, Otsuka Y, Isobe K, Takaso M, Tokunaga M, Nishikawa S, Maruta T, Moriya H: Association between estrogen receptor gene polymorphisms and curve severity of idiopathic scoliosis. Spine (Phila Pa 1976). 2002, 27: 2357-2362. 10.1097/00007632-200211010-00009.View ArticleGoogle Scholar
- Yeung HY, Tang NL, Lee KM, Ng BK, Hung VW, Kwok R, Guo X, Qin L, Cheng JC: Genetic association study of insulin-like growth factor-I (IGF-I) gene with curve severity and osteopenia in adolescent idiopathic scoliosis. Stud Health Technol Inform. 2006, 123: 18-24.PubMedGoogle Scholar
- Dormans JP, Grant SFA, Rendon N, Mentch FD, Kim CE, Frackelton EC, Bradfield JP, Zhang H, Chiavacci RM, Hakonarson H: A genome wide association study identifies an IL17RC missense mutation (S111 L) as an adolescent idiopathic scoliosis locus. 60th Am Soc Hum Genet Meet. 2010, Washington DC: Platform Session 51: Polygenic Traits: GWAS Methods and Results, 99-November 2Google Scholar
- Allen WH, Gaffen SL: IL-17RC: a partner in IL-17 signaling and beyond. Semin Immunopathol. 2010, 32: 33-42. 10.1007/s00281-009-0185-0.View ArticleGoogle Scholar
- Ioannidis JP, Ntzani EE, Trikalinos TA, Contopoulos-Ioannidis DG: Replication validity of genetic association studies. Nat Genet. 2001, 29: 306-309. 10.1038/ng749.View ArticlePubMedGoogle Scholar
- Risser JC: The iliac apophysis: an invaluable sign in the management of scoliosis. Clin Orthop Relat Res. 1958, 11: 111-119.Google Scholar
- Adams W: Lectures on the Pathology and Treatment of Lateral and Other Forms of Curvature of the Spine. 1865, London: ChurchillGoogle Scholar
- Riseborough EJ, Wynne-Davies RA: A genetic survey of idiopathic scoliosis in Boston, Massachusetts. J Bone Joint Surg Am. 1973, 55: 974-982.PubMedGoogle Scholar
- Kesling KL, Reinker KA: Scoliosis in twins. a meta-analysis of the literature and report of six cases. Spine (Phila Pa 1976). 1997, 22: 2009-2014. 10.1097/00007632-199709010-00014.View ArticleGoogle Scholar
- Ohtsuka Y, Yamagata M, Arai S, Kitahara H, Minami S: School screening for scoliosis by the Chiba University Medical School screening program: results of 1.24 million students over an 8-year period. Spine (Phila Pa 1976). 1988, 13: 1251-1257. 10.1097/00007632-198811000-00008.View ArticleGoogle Scholar
- Andrew TH, Mark IMC: What makes a good genetic association study?. Lancet. 2005, 366: 1315-1323. 10.1016/S0140-6736(05)67531-9.View ArticleGoogle Scholar
- Zhang HQ, Lu SJ, Tang MX, Chen LQ, Liu SH, Guo CF, Wang XY, Chen J, Xie L: Association of estrogen receptor beta gene polymorphisms with susceptibility to adolescent idiopathic scoliosis. Spine (Phila Pa 1976). 2009, 34: 760-764. 10.1097/BRS.0b013e31818ad5ac.View ArticleGoogle Scholar
- Takahashi Y, Matsumoto M, Karasugi T, Watanabe K, Chiba K, Kawakami N, Tsuji T, Uno K, Suzuki T, Ito M, Sudo H, Minami S, Kotani T, Kono K, Yanagida H, Taneichi H, Takahashi A, Toyama Y, Ikegawa S: Replication study of the association between adolescent idiopathic scoliosis and two estrogen receptor genes. J Orthop Res. 2011, 29: 834-837. 10.1002/jor.21322.View ArticlePubMedGoogle Scholar
- Neale BM, Sham PC: The future of association studies: gene-based analysis and replication. Am J Hum Genet. 2004, 75: 353-362. 10.1086/423901.View ArticlePubMedPubMed CentralGoogle Scholar
- Takahashi Y, Kou I, Takahashi A, Johnson TA, Kono K, Kawakami N, Uno K, Ito M, Minami S, Yanagida H, Taneichi H, Tsuji T, Suzuki T, Sudo H, Kotani T, Watanabe K, Chiba K, Hosono N, Kamatani N, Tsunoda T, Toyama Y, Kubo M, Matsumoto M, Ikegawa S: A genome-wide association study identifies common variants near LBX1 associated with adolescent idiopathic scoliosis. Nat Genet. 2011, 43: 1237-1240. 10.1038/ng.974.View ArticlePubMedGoogle Scholar
- Deborah AS, Donny LFC, Wilson SC: Interleukin-1β and tumor necrosis factor-α decrease collagen synthesis and increase matrix metalloproteinase activity in cardiac fibroblasts in vitro. Circ Res. 2000, 86: 1259-1265. 10.1161/01.RES.86.12.1259.View ArticleGoogle Scholar
- Sato K, Suematsu A, Okamoto K, Yamaguchi A, Morishita Y, Kadono Y, Tanaka S, Kodama T, Akira S, Iwakura Y, Cua DJ, Takayanagi H: Th17 functions as an osteoclastogenic helper T cell subset that links T cell activation and bone destruction. J Exp Med. 2006, 203: 2673-2682. 10.1084/jem.20061775.View ArticlePubMedPubMed CentralGoogle Scholar
- Kotake S, Udagawa N, Takahashi N, Matsuzaki K, Itoh K, Ishiyama S, Saito S, Inoue K, Kamatani N, Gillespie MT, Martin TJ, Suda : TIL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 1999, 103: 1345-1352. 10.1172/JCI5703.View ArticlePubMedPubMed CentralGoogle Scholar
- Huang H, Kim HJ, Chang EJ, Lee ZH, Hwang SJ, Kim HM, Lee Y, Kim HH: IL-17 stimulates the proliferation and differentiation of human mesenchymal stem cells: implications for bone remodeling. Cell Death Differ. 2009, 16: 1332-1343. 10.1038/cdd.2009.74.View ArticlePubMedGoogle Scholar
- Périé D, Curnier D: Effect of pathology type and severity on the distribution of MRI signal intensities within the degenerated nucleus pulposus: application to idiopathic scoliosis and spondylolisthesis. BMC Musculoskelet Disord. 2010, 26 (11): 189-View ArticleGoogle Scholar
- Qiu Y, Sun X, Qiu X, Li W, Zhu Z, Zhu F, Wang B, Yu Y, Qian B: Decreased circulating leptin level and its association with body and bone mass in girls with adolescent idiopathic scoliosis. Spine (Phila Pa 1976). 2007, 32: 2703-2710. 10.1097/BRS.0b013e31815a59e5.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2474/13/181/prepub
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