Fig. 10

AIF1 suppressed osteosarcoma progression in vitro. A-B Western blot result showing down-regulated AIF1 expression in 143B, MG63, U2OS cell lines compared with hFOB1.19 (osteoblasts cells). C-D The protein expression level of AIF1 after transfected with sh-AIF1 or not was detected by western blot assays. E-F Growth curves were analyzed by cell proliferation assay. G-I Wound-healing assay showed the motility ability. J-K The invasive ability of stable overexpression of RILP in osteosarcoma cells was detected by transwell assay. L-M Immunohistochemical analysis of AIF1 in normal and tumors. Scale bar: 50 μm. N-P Western blot assay was performed to detect the protein expression level of NF-κB, p-NF-κB, BCL-2, and BAX in osteosarcoma cells stably overexpressing AIF1. (**P < 0.01, ***P < 0.001, ****P < 0.0001)