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Fig. 5 | BMC Musculoskeletal Disorders

Fig. 5

From: Knockdown of the UL-16 binding protein 1 promotes osteoblast differentiation of human mesenchymal stem cells by activating the SMAD2/3 pathway

Fig. 5

Suppressed ULBP1 promotes osteoblast differentiation by activating TNF-β signaling pathway. Protein expression of SMAD2/3 as well as phosphorylated SMAD2/3 were measured by western blot assay. B. SMAD2 expression levels were detected by qRT-PCR. C. ALP activity of hMSCs were detected by ALP activity assay. D. mRNA expression levels of BMP2, OCN, and Osterix were measured by qRT-PCR. E. Calcium mineralized level of hMSCs were detected by ARS staining. F. Protein expression levels of BMP2, OCN, and Osterix were measured by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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