Fig. 2From: Knockdown of the UL-16 binding protein 1 promotes osteoblast differentiation of human mesenchymal stem cells by activating the SMAD2/3 pathwayDifferentiated hMSCs reveals the osteoblast differentiation capacity. ALP activity of hMSCs were detected by ALP activity assay. B. mRNA expression levels of BMP2, OCN, and Osterix were measured by qRT-PCR. C. Calcium mineralized level of hMSCs were detected by ARS staining. D. Protein expression levels of BMP2, OCN, and Osterix were measured by western blot assay. **P < 0.01, ***P < 0.001Back to article page