Fig. 5

Suppressed CCNA1 promoted osteogenesis by activating TGF-beta signaling pathway. (A) Protein expression of SMAD2/3 as well as phosphorylated SMAD2/3 measured by western blotting. (B) Image and quantification of Alizarin Red stained MC3T3-E1 cells after LY2109761 treatment. (C) Image and quantification of ALP stained cells MC3T3-E1 cells after LY2109761 treatment. D-E. Expression levels of ALP, OCN, OPN, Osterix, and BMP2 measured by qRT-PCR and western blotting after LY2109761 treatment. **p < 0.01, ***p < 0.001, compared with si-nc group. #p < 0.05, ##p < 0.01, ###p < 0.001, compared with si-CCNA1 group