Fig. 3

CCNA1 knockdown promotes osteogenesis of MC3T3-E1 cells. (A) Detection of CCNA1 expression levels by qRT-PCR after transfection. (B) Image and quantification of Alizarin Red stained MC3T3-E1 cells with inhibited CCNA1. (C) Image and quantification of ALP stained MC3T3-E1 cells with inhibited CCNA1. D-E. Expression levels of ALP, OCN, OPN, Osterix, and BMP2 measured by qRT-PCR and western blotting. **p < 0.01, ***p < 0.001