Fig. 2

Successful establishment of DEX-treated MC3T3-E1 cells as osteoporosis model cells. (A) Image and quantification of Alizarin Red stained DEX-treated MC3T3-E1 cells. (B) Image and quantification of ALP stained DEX-treated MC3T3-E1 cells. C-D. Measurement of the expression levels of ALP, OCN, OPN, osterix, and BMP2 using qRT-PCR and western blotting. **p < 0.01, ***p < 0.001