Figure 6From: Age of donor alters the effect of cyclic hydrostatic pressure on production by human macrophages and osteoblasts of sRANKL, OPG and RANK Effect of hydrostatic pressure on the number of VNR positive cells. MP (5 × 105/ml) from healthy volunteers were cultured at 5 × 105 cells/ml on Thermanox coverslips in supplemented medium for 7 days before pressurisation (34.5 × 10-3MPa) (5.0 psi) for four cycles of pressure. MP were fixed and immunostained for the VNR; a) with pressure; b) without pressure.Back to article page