Figure 4
From: Analyses of the differentiation potential of satellite cells from myoD -/-, mdx, and PMP22 C22 mice
Prolongation of differentiation rescues the satellite cell differentiation defect. Satellite cells of 6 months old mdx or control animals were plated and allowed to proliferate as described in Materials and Methods and then cultivated either for 2 days (2d diff) or for 5 days (5d diff) in differentiation medium. Prolongation of the differentiation period to 5 days enhances differentiation efficiency (* P < 0.0028) of affected mdx satellite cells to the level observed in controls.