Figure 7

Serial sections of soft tissue of 24 months hip NPOA: a: ALP activity counter stained with nuclear red-eosin: Slide 118; Important angiogenesis encircles avascular areas. Many of these vessels express ALP activity (ALP+). b: Alcian bleu pH = 1 staining: Slide 70: chondroitin sulfate accumulation in cartilage. c: ALP activity counter stained with nuclear red-eosin: Slide 71. d: Von Kossa staining countre stained with h&e: Slide 65.