Figure 2

GLPG0492 reduced muscle fibers atrophy induced by hindlimb immobilization. Panel A: representative immuno-fluorescence staining of gastrocnemius cryosections for myosin light chain and laminin discriminating slow-twitch fibers (red star) from fast-twitch fibers (white star). TP and GLPG0492 were tested at 1 and 10 mg/kg/day, respectively. Panel B: FCSA was determined at day 7 in intact and immobilized mice receiving increasing doses of GLPG0492 (0.3, 3, 10 mg/kg/day) or TP (1 mg/kg/day) or CTL vehicle. (n = 10 per group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. intact; ***p < 0.001 vs. CTL immobilized). Panel C: Cross-sectional area (CSA) of gastrocnemius muscle fast and slow fibers distribution (n = 10 per group; *p < 0.05, **p < 0.01, ***p < 0.001 vs. CTL immobilized).