Figure 3

Effects of the selective CB ₂ agonist JWH133 on peripheral blood monocytes. Peripheral blood monocytes were treated with various concentrations of JWH133, from 30 minutes before the stimulation with M-CSF and RANKL. Cells were stained with TRAP (A). TRAP-positive multinucleated cells in a randomly selected field examined at ×40 magnification were counted (B). Representative data (mean ± SEM) from one of three independent experiments analyzed in triplicate are shown. The osteoclasts were incubated on calcium phosphate-coated plates (C). The area of resorption lacunae was examined under light microscopy (D). Representative data (mean ± SEM) from one of three independent experiments analyzed in triplicate are shown. Bar, 400 μm. *p < 0.05, **p < 0.01.