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Figure 3 | BMC Musculoskeletal Disorders

Figure 3

From: Staphylococcus aureus biofilms decrease osteoblast viability, inhibits osteogenic differentiation, and increases bone resorption in vitro

Figure 3

Staphylococcal biofilm factors inhibit osteogenic differentiation in human osteoblasts. A) ALP concentration in osteoblasts exposed to BCM. Bars represent the averages ± std dev from three independent experiments. B) Calcium deposition in osteoblasts and C) osteocalcin in osteoblasts treated with BCM revealed by Alizarin Red S staining and immunofluoresence using specific antibody, respectively. Images were taken at 10× magnification (bars represent 100 μm). Statistical analysis was performed using a One-Way ANOVA analysis using a Bonferroni test to determine statistical differences between groups.* p< 0.01 versus control group.

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