Figure 6

OCN, Cbfα1 mRNA expression of irradiated MC3T3-E1 cells. MC3T3-E1 cells were exposed to 0, 0.1, 0.5 and 1.0 Gy of X-irradiation respectively and cells were harvested on day 10 after irradiation. Total RNA was collected and real-time PCR was performed, β-actin as an endogenous control. Results were expressed as fold change over the control group (0 Gy). (A) OCN mRNA expression in 0.5 and 1 Gy groups were significantly increased compared with the control group (*p < 0.05, **p < 0.01). (B) Cbfα1 mRNA expression in 0.5 and 1 Gy groups were significantly increased compared with the control group (*p < 0.05).