Fig. 1

Experimental Design. Ovariectomy (OVX) and tibial osteotomy (distraction osteogenesis [DO] surgery) were performed at the ages of 8 and 16 weeks, respectively. After osteotomy, a 5-day-latency phase was followed by distraction at a rate of 0.2 mm/day for 9 days. Day 0 was defined as the day of distraction completion. Closed circles indicate the time points for each analysis including bone mineral density, soft X-ray, micro-computed tomography (CT), blood test, and histology