Fig. 6

Effects of treadmill training on the NLRP3/Caspase1/IL-1β signaling in bone marrow in aged rats. The cropped gels and blots by western blot of proteins levels of NLRP3, proCaspase1, cleaved Caspase1, IL-1β, GSDMD-N, and β-actin A. Quantitative analysis of NLRP3 (B), proCaspase1 (C), cleaved Caspase1 (D), IL-1β (E), and GSDMD-N (F) in the different groups; The mRNA expression of NLRP3 (G), Casepase1 (H), and IL-1β (I) was detected by qRT-PCR. Data are expressed as mean ± S.E.M (n = 6 per group). *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001 vs. YC group. #P<0.05, ##P<0.01, ###P<0.01, and ####P<0.0001 vs. AC group. YC, adult rats with sham training; AC, aged rats with sham training; AT, aged rats with real training. All the samples in this figure are derived from the same experiment and the gels/blots were processed in parallel