Fig. 7

PKM2 inhibition reduce the cell apoptosis induced by ATF4 silence under the nutritional deficiency condition by inhibiting AKT phosphate

(A) The expression level of cell apoptosis protein detected by western blot induced by ATF4 silence or PKM2 inhibition under the nutritional deficiency condition (Group A, B, C, D, E denotes the 10%FBS, 1%FBS, 1%FBS with siATF4, 1%FBS with PKM2-IN-1, 1%FBS + siATF4 + PKM2-IN-1 group respectively) (B) The quantity analysis of expression level of cell apoptosis protein as showed in A (C)The AKT and P-AKT expression level detected by western blot induced by ATF4 silence or PKM2 inhibition under the nutritional deficiency condition (Group A, B, C, D, E denotes the 10%FBS, 1%FBS, 1%FBS with siATF4, 1%FBS with PKM2-IN-1, 1%FBS + siATF4 + PKM2-IN-1 group respectively) (D) The quantity analysis of expression level of cell apoptosis protein as showed in C. Data are mean ± s.d. The percentage calculation in each tissue were measured at least 1000 cells each sample. Statistical significance was determined by one-way ANOVA and Student’s t-test. **, ##, △△ P < .001