Fig. 2

ATF4 silence can induce NP cell’s apoptosis under the nutritional deficiency condition

(A) ATF4 expression level detected by RT-qPCR (B) ATF4 expression level detected by western blot (C) The quantity analysis of expression level of ATF4 as showed in B (D) The NP cell proliferation status detected by CCK8 under the condition of nutritional deficiency and ATF4 silence (E)The cell apoptosis status detected by flow cytometry under the condition of nutritional deficiency and ATF4 silence (F) The quantity data showed percentage of cells in apoptosis as showed in E (G) The cell apoptosis protein detected by western blot (Group A, B, C denotes the 10%FBS, 1%FBS and 1%FBS with siATF4 group respectively) (H) The quantity analysis of expression level of cell apoptosis protein as showed in G. Data are mean ± s.d The percentage calculation in each tissue were measured at least 1000 cells each sample. Statistical significance was determined by one-way ANOVA and Student’s t-test. **, ##, △△ P < .001