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Table 1 The primers used for qRT-PCR analysis

From: MiR-99a alleviates apoptosis and extracellular matrix degradation in experimentally induced spine osteoarthritis by targeting FZD8

Genes

Forward primer (5′-3')

Reverse primer (5’-3’)

GAPDH

TGTTCGTCATGGGTGTGAAC

ATGGCATGGACTGTGGTCAT

FZD8

AGTGGGGTTACCTGTTGGAA

ACTGATTGGGCATGTAGGTG

U6

CTCGCTTCGGCAGCACA

AACGCTTCACGAATTTGCGT

hsa-miR-99a

ACACTCCAGCTGGGAACCCGTAGATCCGATCT

CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCACAAGAT

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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