Skip to main content
Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: MiR-99a alleviates apoptosis and extracellular matrix degradation in experimentally induced spine osteoarthritis by targeting FZD8

Fig. 4

FZD8 overexpression reversed the effects of miR-99a on ECM degradation and apoptosis. SW1353 cells were transfected with the vector, miR-99a agomir alone, or co-transfected with the miR-99a agomir and FZD8 overexpression plasmid, followed by treatment with IL-6 and TNF-α for 48 h. (A) CCK-8 assays were performed to evaluate the viability of transfected SW1353 cells. (B-C) The levels of miR-99a and FZD8 expression were determined in transfected SW1353 cells. (D) The levels of Bax, Bcl-2, caspase-3, Col2A1, MMP-13, β-catenin, and FZD8 proteins were determined by western blotting. (E) The levels of FZD8 protein in transfected SW1353 cells were detected by immunofluorescence. (F) The apoptosis of transfected SW1353 cells was analyzed by flow cytometry. ***p < 0.001, compared with vector; ###p < 0.001, compared with miR-99a, N = 3

Back to article page