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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: MiR-99a alleviates apoptosis and extracellular matrix degradation in experimentally induced spine osteoarthritis by targeting FZD8

Fig. 3

Overexpression of miR-99a attenuated IL-6/TNF-α‐induced ECM degradation and cell apoptosis. SW1353 cells were transfected with the miR-99a agomir, antagomir or scramble and then treated with IL-6 and TNF-α for 48 h. (A) CCK-8 assays were performed to evaluate the viability of transfected SW1353 cells. (B) The levels of miR-99a and FZD8 expression in transfected SW1353 cells. (C) The levels of Bax, Bcl-2, caspase-3, Col2A1, MMP-13, β-catenin, and FZD8 proteins in transfected SW1353 cells were measured by western b lotting. (D) FZD8 protein expression was detected by immunofluorescence. (E) SW1353 cell apoptosis was analyzed by flow cytometry. (F) The levels of MMP13, Col2A1, and FZD8 expression in SW1353 cells transfected with the scramble or antagomir, with or without IL-6/TNF-α stimulation. ***p < 0.001, compared with control + scramble; ##p < 0.01, ###p < 0.001, compared with IL-6 and TNF-α + scramble, N = 3

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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