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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: MiR-99a alleviates apoptosis and extracellular matrix degradation in experimentally induced spine osteoarthritis by targeting FZD8

Fig. 2

FZD8 was a potential target of miR-99a. (A) The viability of SW1353 cells was determined after 0, 12, 24, 36, 48, and 56 h of treatment with 10 ng/mL IL-6 and TNF-α. (B) SW1353 cell apoptosis was analyzed by flow cytometry. (C) The levels of Bax, Bcl-2, caspase-3, Col2A1, MMP-13, β-catenin, and FZD8 proteins as determined by western blotting. (D) The levels of miR-99a and FZD8 mRNA in SW1353 cells were determined by quantitative real time PCR. (E) The predicted binding sites between miR-99a and the 3′-UTR of FZD8 mRNA. (F) The targeting effect of miR-99a and FZD8 was measured by a luciferase reporter assay. (G) The levels of FZD8 protein in SW1353 cells were detected by immunofluorescence. (H) FZD8 expression in mild OA cartilage tissue and severe OA cartilage tissue as determined by western blotting. ***p < 0.001, compared with control or NC, N = 3

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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