Fig. 4

Treatment with chitosan (Cs) and curcumin (Cn) NPs decreased fibrosis index in glycerol-injured rat muscles. a Light micrographs of rat tibialis anterior (TA) muscles from non-injured muscle (negative control), glycerol-injured (positive control), muscle treated with Cs-50, − 200, and − 400 μg, and Cn-5, − 20, − 40 μg. Sirius red stain. b Quantification of Sirius red-positive area. Data are expressed as means ± SD, * indicates significant difference from glycerol-injured muscle, # indicates significant difference between treatment groups (P < 0.05)