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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: Low-frequency electrical stimulation alleviates immobilization-evoked disuse muscle atrophy by repressing autophagy in skeletal muscle of rabbits

Fig. 2

Immobilization induced activation of autophagy in rabbit skeletal muscle. The left hind limb of male rabbits was immobilized with a plaster bandage on the left knee joint in the extension position. The rectus femoris was dissected at 2, 4, and 6 weeks after immobilization. A Western blotting of mTOR, p-mTOR, and Atg7 proteins. B Quantitative analysis of mTOR. C Quantitative analysis of p-mTOR. D Quantitative analysis of Atg7. E Western blotting of LC3B-I/II protein. F Quantitative analysis of LC3B-II. G Western blotting of p62 protein. H Quantitative analysis of p62. Ctrl1: control 1 group; I-2: immobilization for 2 weeks; I-4: immobilization for 4 weeks; I-6: immobilization for 6 weeks; mTOR, mammalian target of rapamycin; p-mTOR, phosphorylated mammalian target of rapamycin; Atg7, autophagy-related protein 7; LC3B, microtubule-associated protein light chain 3B. Data are expressed as mean ± SD. n = 6. *P < 0.05, **P < 0.01 compared with the Ctrl1 group; &P < 0.05, &&P < 0.01 compared with the I-2 group; ##P < 0.01 compared with the I-4 group

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