Fig. 4

Inhibitory effect of Bcat1 knockdown on activation of the mTORC1-S6K1 signal pathway. A Immunoblot analysis of mTOR, p-mTOR, pS6K1, and S6K1 in mouse C2C12 cells. Grouping: control vector (shCtrl), shBcat1, shBcat1 + mTOR activator, shBcat1 + shRNA-resistant Bcat1 cDNA (rescue), shBcat1 + shRNA-resistant Bcat1 cDNA (rescue) + mTOR inhibitor, n = 3, cells were analyzed at 24 h post-treatment. Representative images of protein levels of mTOR, p-mTOR, S6K1, and pS6K1 in muscle cells are presented. B-C Quantitative analysis of protein levels of p-mTOR and pS6K1, where the relative band intensity was normalized to GAPDH. Data from three independent experiments are presented as mean + SEM. *p < 0.05, **p < 0.01