Fig. 2

Orai1 downregulation reduces the proliferation of MC3T3-E1 cells and causes their cell cycle arrest. Control siRNA and Orai1 siRNA transfected into MC3T3-E1 cells for an appropriate period. The 2-APB stock solution was diluted to a concentration of 50 μM in the cell culture medium. DMSO alone was dissolved as negative (vehicle) control. After that, cell proliferation and cell cycle assays were performed at an appropriate period. a The cell proliferation was evaluated by the MTT assay of MC3T3-E1 cells transfected with either control siRNA, Orai1 siRNA, DMSO or 2-APB. b Apoptosis was evaluated by Tunel staining of the MC3T3-E1 cells transfected with either control siRNA or Orai1 siRNA (Scale =100 μm). c The cell cycle distribution was investigated using PI staining of the MC3T3-E1 cells transfected with either control siRNA, Orai1 siRNA, DMSO or 2-APB. (Data are presented as means ± SD from three independent experiments. *p < 0.05 as compared with the control