Fig. 5

RvD1 inhibits the expression of MMP-13, and IL-1β and promotes YAP phosphorylation. A MMP-13 was measured by western blot analysis in OA-FLSs after incubation with RvD1 (0, 20, and 200 nM) for 48 h. B IL-1β was measured by ELISA in OA-FLSs after incubation with RvD1 (0, 20, and 200 nM) for 48 h. C YAP was detected by immunofluorescence staining in OA-FLSs after incubation with RvD1 (0, 20, and 200 nM) for 48 h (400 × magnification). D LATS1, p-YAP, and YAP were measured by western blot analysis in OA-FLSs after incubation with RvD1 (0, 20, and 200 nM) for 48 h. *P < 0.05; **P < 0.01 as comparison with OA-FLS group. All experiments were repeated three times independently