Fig. 2

The presented western blots of all samples tested for acrolein-lysine adduct (A), MMP2 (B) and the loading control, A1AT (C). The molecular weight of the bands are shown in kilodaltons (kDa) on the right at each row. The images are of the cut strips of nitrocellulose membrane used as a sample sparing strategy when performing western blots. The highlighted (yellow) samples from four dogs (two OA and one control) were excluded from analysis in the study. These four samples had been obtained from the joints contralateral to those included in the study that were also affected with OA but the study required only one sample per dog to be included in the analysis. OA Osteoarthritis group sample, CtL Control group sample, L molecular ladder, MMP2 matrix metalloproteinase, A1AT α-1 antitripsin