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Fig. 5 | BMC Musculoskeletal Disorders

Fig. 5

From: Rolipram plays an anti-fibrotic effect in ligamentum flavum fibroblasts by inhibiting the activation of ERK1/2

Fig. 5

Rolipram restores ERK1/2 signaling to block TGF-β1 stimulated LF fibroblast fibrosis. (A) Different drugs were used to treat fibroblasts stimulated by TGF-β1. WB detects the expression of TGF-β1. a, Rolipram (50 nM) is a PDE4 inhibitor.; b, WAY-262611 (1 μM) is the Wnt/β-catenin pathway activator.; c, LY294002 (1 μM) is an inhibitor of the PI3K pathway; d, SCH772984 (1 μM) inhibits ERK1/2 signal transduction. (B) The expression of FN1 and TGF-β1 was up-regulated after exogenous TGF-β1 stimulation. Using Rolipram or SCH772984 blocked this change. (C) Western blot analysis reveals the expression levels of p-ERK1/2 and ERK1/2 in LF fibroblasts treated with TGF-β1 (5 ng/ mL) and Rolipram (50 nM, 24 h). β-tubulin served as an endogenous loading control. *p < 0.05

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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