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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Norepinephrine modulates IL-1β-induced catabolic response of human chondrocytes

Fig. 3

β-AR blockers affect IL-1β-induced expression of catabolic and anabolic factors. (A), (B) The effect of β-AR blockers on IL-1β-induced expression of (A) matrix degrading enzymes and (B) extracellular matrix proteins. Chondrocytes were pre-incubated with 0.1, 1.0, and 10 ng/ml of propranolol (P; non-selective β-adrenergic blocker) and atenolol (A; β1-adrenergic blocker) for 2 h followed by treatment with IL-1β (1 ng/ml) for 6 h. The mRNA levels of matrix degrading enzymes (MMP-1, -3, and − 13) and extracellular matrix proteins (ACAN and Col II) were measured using RT-PCR. Data represent the mean ± SD of duplicate data from three different donors. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. IL-1β-treated cells by Kruskal-Wallis test with post hoc Dunn’s multiple comparison test

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