Fig. 1From: TGF-β regulates nerve growth factor expression in a mouse intervertebral disc injury modelGeneration of Tnfa knockout mice. A Schematic showing the structure and map of the mouse Tnfa locus. The location of the two gRNAs and primer sequences for polymerase chain reaction (PCR) are shown. B PCR screen for Tnfa-KO founder mice. C The gene sequence at the junction of the deleted alleles. D Confirmation of loss of TNF-α protein by ELISA (n = 6)Back to article page