Table 3 Methylation levels of the OPG, RANK and RANKL genes

OPG1_CpG_2

49

50

0.06 ± 0.01

0.07 ± 0.01

0.046 ^a

OPG2_CpG_1

50

49

0.08 ± 0.09

0.13 ± 0.10

0.016 ^b

OPG2_CpG_5

47

47

0.05 ± 0.04

0.07 ± 0.06

0.032 ^a

OPG2_CpG_10.11

48

50

0.04 ± 0.02

0.05 ± 0.03

0.020 ^b

RANK1_CpG_6

48

49

0.16 ± 0.04

0.09 ± 0.08

0.035 ^a

RANK1_CpG_15

49

50

0.82 ± 0.25

0.93 ± 0.20

0.001 ^a

RANK1_CpG_20

49

50

0.07 ± 0.09

0.12 ± 0.15

0.005 ^a

RANK1_CpG_21

48

47

0.13 ± 0.17

0.23 ± 0.22

0.044 ^a

RANK2_CpG_10.11

46

50

0.07 ± 0.04

0.17 ± 0.19

< 0.001 ^a

RANK2_CpG_15

47

50

0.04 ± 0.04

0.09 ± 0.08

0.001 ^a

RANK2_CpG_16

46

48

0.05 ± 0.03

0.08 ± 0.02

< 0.001 ^a

RANK2_CpG_21.22

47

50

0.02 ± 0.01

0.03 ± 0.03

0.043 ^a

RANK2_CpG_23.24.25.26.27

47

50

0.04 ± 0.02

0.07 ± 0.03

< 0.001 ^a

RANK2_CpG_29

47

50

0.01 ± 0.02

0.03 ± 0.04

0.007 ^a

RANK2_CpG_30.31

45

49

0.04 ± 0.02

0.05 ± 0.01

< 0.001 ^a

RANK2_CpG_36

47

50

0.03 ± 0.03

0.04 ± 0.03

0.019 ^a

RANK3_CpG_1

46

50

0.38 ± 0.09

0.41 ± 0.07

0.023 ^a

RANK3_CpG_8

46

50

0.05 ± 0.03

0.09 ± 0.00

< 0.001 ^b

RANK3_CpG_10

47

50

0.03 ± 0.02

0.04 ± 0.01

0.007 ^a

RANKL1_CpG_1

43

49

0.07 ± 0.05

0.12 ± 0.11

0.009 ^b

RANKL1_CpG_11

45

50

0.02 ± 0.03

0.03 ± 0.02

0.014 ^a

RANKL1_CpG_12.13

44

49

0.10 ± 0.07

0.11 ± 0.03

0.004 ^a

RANKL1_CpG_19

43

49

0.07 ± 0.15

0.10 ± 0.14

0.001 ^a

RANKL1_CpG_21

44

50

0.06 ± 0.08

0.08 ± 0.14

0.041 ^a

RANKL1_CpG_24

46

50

0.11 ± 0.15

0.13 ± 0.16

0.018 ^a

RANKL1_CpG_25.26.27

45

50

0.30 ± 0.19

0.35 ± 0.15

< 0.001 ^a

RANKL1_CpG_44.45

45

50

0.03 ± 0.01

0.04 ± 0.01

< 0.001 ^b

RANKL2_CpG_2

48

50

0.10 ± 0.06

0.15 ± 0.09

0.016 ^b

RANKL2_CpG_9.10.11.12

48

50

0.05 ± 0.04

0.07 ± 0.02

< 0.001 ^a