DNA methylation in the OPG/RANK/RANKL pathway is associated with steroid-induced osteonecrosis of the femoral head

Table 1 Primers used for this study

Gene_ID	Direction	Primer (5′ → 3′)	Product	Number of
Gene_ID	Direction	Primer (5′ → 3′)	Length (bp)	CG
OPG1	F	aggaagagagTTTTTGTTGTTTTTTATAAAGTTAGTAGGA	185	6
OPG1	R	cagtaatacgactcactatagggagaaggctACTACTACCACCTAATCTCCCAACC	185	6
OPG2	F	aggaagagagGAAAGGTGTAAAGTTTGGTTTAGGA	265	21
OPG2	R	cagtaatacgactcactatagggagaaggctAAAAAACCAAATAACAACAACCTCC	265	21
RANK1	F	aggaagagagAAGAAAAAGAGATAGTGGTTGTTGGT	464	33
RANK1	R	cagtaatacgactcactatagggagaaggctCAAATAATACCCAAACTCCCCTAAT	464	33
RANK2	F	aggaagagagGGTTTTGATGTTGTTATTTTTTTTAAATGT	491	38
RANK2	R	cagtaatacgactcactatagggagaaggctCCTTCCCTATAAAAACTTTCAAATTC	491	38
RANK3	F	aggaagagagATTTGAAAGTTTTTATAGGGAAGGG	349	12
RANK3	R	cagtaatacgactcactatagggagaaggctAAACACTTAATTAAACAACACCTAAAA	349	12
RANKL1	F	aggaagagagTAGAGGTGGGAGTGGAAGAGGTAGTT	497	46
RANKL1	R	cagtaatacgactcactatagggagaaggctATCCCCTAAAAAAATAACCACTCAC	497	46
RANKL2	F	aggaagagagGATTTTTTGGGAAGGTGGTTATTTA	235	19
RANKL2	R	cagtaatacgactcactatagggagaaggctCCAACAAAAACTACACCAAATACCT	235	19

ISSN: 1471-2474