Table 1 Primer sequences used for polymerase chain reaction
Gene | Oligonucleotide sequence |
|---|---|
NOX1 | Forward 5’ GTGGCTTTGGTTCTCATGGT 3’ Reverse 5’ TGAGGACTCCTGCAACTCCT 3’ |
NOX4 | Forward 5’ GGGCCTAGGATTGTGTTTGA 3’ Reverse 5’ CTGAGAAGTTCAGGGCGTTC 3’ |
Type I collagen | Forward 5’ TGGAGACAGGTCAGACCTG 3’ Reverse 5’ TATTCGATGACTGTCTTGCC 3’ |
Type III collagen | Forward 5’ TAAAGGGTGAACGGGGCAGT 3’ Reverse 5’ ACGTTCCCCATTATGGCCAC 3’ |
MMP-2 | Forward 5’ GGAAGCATCAAATCGGACTG 3’ Reverse 5’ GGGCGGGAGAAAGTAGCA 3’ |
TIMP-1 | Forward 5’ ATAGTGCTGGCTGTGGGGTGTG 3’ Reverse 5’ TGATCGCTCTGGTAGCCCTTCTC 3’ |
TIMP-2 | Forward 5’ GGACACGCTTAGCATCACCCAGA 3’ Reverse 5’ GTCCATCCAGAGGCACTCATCC 3’ |
IL-6 | Forward 5’ GGTCTTCTGGAGTTCCGTTTC 3’ Reverse 5’ GGTCTTGGTCCTTAGCCATCT 3’ |
GAPDH | Forward 5’ GGTGGTCTCCTCTGACTTCAACA 3’ Reverse 5’ GTTGCTGTAGCCAAATTCGTTGT 3’ |