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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: In vitro and in vivo tenocyte-protective effectiveness of dehydroepiandrosterone against high glucose-induced oxidative stress

Fig. 4

Immunofluorescence staining for analysis of apoptotic cells. (A) Immunofluorescence staining showing all cells (blue; DAPI) and apoptotic cells (green; APO-direct, white arrow (→)). There were few apoptotic cells in the low-glucose without dehydroepiandrosterone (LG DHEA–) and with DHEA (LG DHEA+) groups. There was induction of apoptosis in the high-glucose (HG) groups. The number of apoptotic cells in the HG DHEA + group was lower than that in the HG DHEA– group. (B) The number of apoptotic cells was analyzed by fluorescence intensity normalized to cell number. The number of apoptotic cells in the high-glucose without dehydroepiandrosterone (HG DHEA–) group was significantly higher than that in low-glucose without DHEA (LG DHEA–) at 48 hours. The number of apoptotic cells in HG DHEA + group was significantly decreased compared with that in HG DHEA– group. *p < 0.05. DAPI, 4’,6-diamidino-2-phenylindole

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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