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Table 1 Sequences of PCR primers for specific genes

From: Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model

Gene   Sequences
β-actin Forward 5′-GGAGATTACTGCCCTGGCTCCTA-3’
Reverse 5′-GACTCATCGTACTC CTGCTTGCTG-3’
Tnf-α Forward 5′-CCTGTAGCCCACGTCGTAG-3’
Reverse 5′-GGGAGTAGACAAGGTACAACCC-3’
Il-1β Forward 5′-GGAGATTACTGCCCTGGCTCCTA-3’
Reverse 5′-GACTCATCGTACTCCTGCTTGCTG-3’
Il-6 Forward 5′-GACAAAGCCAGAGTCCTTCAGA-3’
Reverse 5′-GTCTTGGTCCTTAGCCACTCC-3’
Cd16 Forward 5′-CAGAATGCACACTCTGGAAGC-3’
Reverse 5′-GGGTCCCTTCGCACATCAG-3’
Cd206 Forward 5′-GGAAACGGGAGAACCATCAC-3’
Reverse 5′-GGCGAGCATCAAGAGTAAAG-3’
Mcsf Forward 5′-AACAGCTTTGCTAAGTGCTCTA-3’
Reverse 5′-ACTTCCACTTGTAGAACAGGAG-3’
Mcp-1 Forward 5′-TTTTTGTCACCAAGCTCAAGAG-3’
Reverse 5′-TTCTGATCTCATTTGGTTCCGA-3’
Il-4 Forward 5′-AGT GAG CTC GTC TGT AGGGC-3’
Reverse 5′-CAGGCA TCG AAA AGC CCG AA-3’
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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