Fig. 6

Impaired ostealmacrophage formation in the OVX mice during fracture healing. a Immunohistochemistry staining for F4/80+Mac-2− macrophages with serial sections of the fracture haematoma/callus from OVX and sham mice. Arrows, macrophages with F4/80 positive staining and Mac-2 negative staining. b Quantifications of the numbers of F4/80+Mac-2− macrophages within the periosteal callus or endochondral ossification callus. An average callus area of 0.1 mm2 was assessed. **, p < 0.01 compared with the sham group. n = 6/group