Fig. 2

Impaired endochondral ossification and callus remodeling in OVX mice. a ABH/OG staining of the callus in sham and OVX mice at different time points of fracture healing. Scale bars, 500 μm. b Quantification of the percentages of cartilage area in total callus area. *, p < 0.05 compared with the sham group. n = 6/group. OVX, ovariectomized; ABH/OG, alcian blue hematoxylin/orange G. DPF, days post-fracture