Table 1 Primers used for quantitative RT-PCR experiments
From: Influence of Angptl1 on osteoclast formation and osteoblastic phenotype in mouse cells
Gene | Sequence | |
|---|---|---|
Angptl1 | Forward | CGACACAGTCCTAACAGCCACCAG |
Reverse | TGACAGTCTTTGAATGGTCCTTC | |
TRAP | Forward | CAGCTGTCCTGGCTCAAAA |
Reverse | ACATAGCCCACACCGTTCTC | |
Ctsk | Forward | GAGGGCCAACTCAAGAAGAA |
Reverse | GCCGTGGCGTTATACATACA | |
NFATc1 | Forward | CAAGTCTCACCACAGGGCTCACTA |
Reverse | GCGTGAGAGGTTCATTCTCCAAGT | |
MMP-9 | Forward | GGACCCGAAGCGGACATTG |
Reverse | CGTCGTCGAAATGGGCATCT | |
DC-STAMP | Forward | CTAGCTGGCTGGACTTCATCC |
Reverse | TCATGCTGTCTAGGAGACCTC | |
Runx2 | Forward | AAATGCCTCCGCTGTTATGAA |
Reverse | GCTCCGGCCCACAAATCT | |
Osterix | Forward | AGCGACCACTTGAGCAAACAT |
Reverse | GCGGCTGATTGGCTTCTTCT | |
ALP | Forward | ATCTTTGGTCTGGCTCCCATG |
Reverse | TTTCCCGTTCACCGTCCAC | |
Osteocalcin | Forward | CCTGAGTCTGACAAAGCCTTCA |
Reverse | GCCGGAGTCTGTTCACTACCTT | |
PPARγ | Forward | CCCAATGGTTGCTGATTACAAA |
Reverse | AATAATAAGGTGGAGATGCAGGTTCT | |
aP2 | Forward | CTTCAAACTGGGCGTGGAA |
Reverse | CCATCTAGGGTTATGATGCTCTTCA | |
GAPDH | Forward | ACGGCAAATTCAACGGCAC |
Reverse | CTCCACGACATACTCAGCAC | |
β-actin | Forward | TACCACAGGCATTGTGATGG |
Reverse | TTTGATGTCACGCACGATTT |