Fig. 5

Effects of Angptl1 on adipogenic differentiation. a Total RNA was extracted from transiently empty vector- or Angptl1-transfected 3T3-L1 cells with and without adipogenic medium (10 μg/ml insulin, 500 μM 3-isobutyl-1-methyl-xanthine and 1 nM dexamethasone) for 6 days. Then, qRT-PCR for PPARγ, aP2, Angptl1 or β-actin was performed. Data are expressed relative to β-actin mRNA value. Data represent mean ± SEM (n = 6 in each group). *p < 0.05, **p < 0.01, by Tukey-Kramer test. b Transiently empty vector- or Angptl1-transfected 3T3-L1 cells with and without adipogenic medium for 6 days. Then, cells were stained with Oil red O. Data shows the representative images. Scale bar indicates 50 μm. At least three independent experiments were performed