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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Influence of Angptl1 on osteoclast formation and osteoblastic phenotype in mouse cells

Fig. 3

Effects of Angptl1 on osteoblastic differentiation. a Total RNA was extracted from transiently empty vector- or Angptl1-transfected ST2 cells cultured with vehicle or BMP-2 (200 ng/ml) for 72 h. Then, qRT-PCR for Runx2, Osterix, osteocalcin, Angptl1 or GAPDH was performed. Data are expressed relative to GAPDH mRNA value. Data represent mean ± SEM (n = 6 in each group). *p < 0.05, **p < 0.01 by Tukey-Kramer test. ##p < 0.01 by Mann-Whitney U test. b Cell lysates were extracted from transiently empty vector- or Angptl1-transfected ST2 cells cultured with vehicle or BMP-2 (200 ng/ml) for 72 h. Then, ALP activity was measured as described in Methods. Data represent mean ± SEM (n = 6 in each group). *p < 0.05, **p < 0.01 by Tukey-Kramer test. c Stably- empty vector- or Angptl1 (#1, #2)-transfected ST2 cells were treated with BMP-2 (200 ng/ml) and G418 (300 μg/ml) for 1 week, then cultured with the mineralization medium (10 mM β-glycerophosphate and 50 μg/ml ascorbic acid) and G418 (300 μg/ml) for additional 10 days in the absence of BMP-2. The quantitation of mineralization was performed at the absorbance at 550 nm, as described in Materials and Methods. Data were expressed as ratio of the absorbance in empty vector-overexpressed ST2 cells. Data represent mean ± SEM (n = 8 in each group). **p < 0.01 by Dunnett test. Lower panel shows the representative images of mineralization

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