Fig. 1

Effects of Angptl1 on osteoclast formation in RAW 264.7 cells. a Transiently empty vector- or Angptl1-transfected RAW 264.7 cells were cultured with or without 50 ng/mL RANKL for 5 days. Then, the number of TRAP-positive MNCs was counted. Data represent mean ± SEM (n = 5 in each group). **p < 0.01 by Tukey-Kramer test. b Total RNA was extracted from transiently empty vector- or Angptl1-transfected RAW 264.7 cells cultured with or without 50 ng/mL RANKL for 5 days. Then, qRT-PCR analysis for TRAP, cathepsin K (Ctsk), NFATc1, Angptl1 or GAPDH was performed. Data are expressed relative to GAPDH mRNA value. Data represent mean ± SEM (n = 6 in each group). *p < 0.05, **p < 0.01 by Tukey-Kramer test